(11814460001 . No. Experimental Design and Results Summary Applications Western Blot Sample Hela cells, cell lysate Primary Incubation 1:2000 Milk 5% in PBS 0,05% tween 1h RT Blocking Agent Milk 5% in PBS 0,05% tween Secondary Incubation The Col-0, GFP-rSPL9 transgenic plants and anti-ABI5 antibody were used in the Co-IP assays for the interaction of SPL9 and ABI5. no. Stain free staining was used as loading control. We added 20 M MG132 or an equivalent volume of DMSO (as a control) at 12 h before sampling. Discover More. T-9026) was purchased from . Asked 13th Jul, 2015; Jyoti Iyer; Mouse anti-GFP (#11814460001) was purchased from Roche. ACTIN serves as an internal control. Introduction. Switch Edition. Expand All Collapse. Product Certifications . For anti-green fluorescent protein (GFP) immunoprecipitation, 2 g anti-GFP monoclonal antibody (11814460001, Roche), and 30 l of G-Sepharose protein beads (GE Healthcare Bio-Sciences) were added to each lysate. We celebrate the students, the researchers, the managers who work tirelessly in the pursuit of discovery. 9. Monoclonal anti-GFP mouse IgG (Roche, 11814460001, 1:5000), monoclonal anti-mCherry Rat IgG (Thermo Fisher Scientific, M11217. Signals were visualized by chemiluminescence (SuperSignal West Femto; 34095, Pierce) using secondary antibodies coupled to . Western blot analysis of nuclear lysates prepared from a K562 stable cell line expressin eGFP-ID3 using the Roche anti-GFP 11814450001 antibody. . ab14955; Abcam) and a mouse monoclonal anti-brillarin (cat. Anti-GFP is a mixture of two clones (7.1 and 13.1). Selective autophagic degradation of mitochondria (mitophagy) is important in maintaining proper cellular homeostasis. Incubate the membrane with the primary antibody for 1 h at RT with gentle agitation. anti-GFP antibody (cat. No rating or validation information has been found for Anti-GFP. Anti-rabbit IgG peroxidase antibody produced in goat: Sigma: Cat# A6154, RRID:AB_258284: Anti-mouse IgG peroxidase antibody produced in goat: Blots were probed o/n with primary mouse anti-GFP mAb (11814460001; Roche) at a 1:2.000 dilution, followed by the secondary antibody anti-mouse IgG at a 1:2.000 dilution (A3562; Sigma-Aldrich). Info: this is a Roche product sold by Sigma. If not, could you please suggest an antibody that detects sfGFP with relatively high sensitivity by . SDS. Has anyone used the Roche anti-GFP Antibody (Cat#11814460001) to detect sfGFP on a western blot? Mouse anti-GFP (#11814460001) was purchased from Roche. GFP-tagged protein was detected with anti-GFP (11814460001, Roche). GFP antibody has been used to detect the GFP signal after the transfection of an empty-GFP plasmid in Hela cells. . 10 M Alk12 was infiltrated twice, 24 h after Agrobacterium infiltration and 6 h before sampling. Most recent articles: {{ mention._source.dc.creators[0 . Anti-GFP 11814460001 from Roche | Biocompare: The Buyer's Guide for Life Scientists in Antibodies in Biomolecules in ELISA Kits Anti-GFP from Roche This product is no longer available on Biocompare. Mouse monoclonal anti-GFP: Roche: Cat# 11814460001, RRID:AB_390913: Mouse monoclonal anti-SC35: Abcam: Cat# ab11826,RRID:AB_298608: Mouse monoclonal anti-H2AX: Millipore: . No alerts have been found for Anti-GFP. S2, A and F, bands 4 and 5) as cleavage products downstream of R69 (table S2). . For both Western blots and coimmunoprecipitations, proteins were transferred overnight to nitrocellulose membranes and blotted with either mouse anti-GFP (11814460001; Roche, Basel, Switzerland) or mouse anti-HA (MMS-101R; Covance, Princeton, NJ). Academic Edition; Corporate Edition; Yeast two-hybrid analysis. We report the . No. I am using Roche anti-GFP antibody (Cat#11814460001) for detection of my gene of interest in Hela cells using western blotting. Secondary antibodies were anti-mouse or anti-rabbit HRP conjugates (Dianova, 115-035-003, 111-035-003) and were read out using chemiluminescence. $759.11 / Each; Qty Add to cart Description; SDS; Documents; Product Certifications; Promotions; Description. We identified that neither Aurora B protein Natural Inhibitors targets levels nor Aurora B activity, as measured by autophosphorylation on T232 or phosphorylation of the . Roche: 4905318001: Anti-His6 (2) 0.2 ml Roche: 11814460001: Anti-GFP: 200 g Roche: 11645692001: StreptaWell, HB (transp.12x8 w: 5 plates (12 x 8 well strips and frame) Roche: 11112597001: ABTS Buffer, 16.7g: 16.7 g (for 1 l) Roche: 11112422001: ABTS Tablets, 50 mg: 20 tablets Roche: 11365169001: N-Glycosidase F, 100 u: 100 U (0.1 ml) Roche . Here, we found that SPATA33 is a novel autophagy mediator for mitophagy in testis. Over 10 million scientific documents at your fingertips. The H2A-pT120-Sgo1 pathway serves as an adaptor to facilitate Aurora B inner centromeric accumulation635. ab4566; Abcam). Produced commercially for the modENCODE Project by K. White's group and Millipore. Anti-GFP is a mixture of two clones (7.1 and 13.1). ACT (1:5000; CWBIO, CW0264) was employed as a loading control. The correct assembly of the myotendinous junction (MTJ) is crucial for proper muscle function. Indirect immunofluorescence and fluorescence in situ hybridisation They work amazingly well. Roche mouse monoclonal anti-GFP antibody (clone 7.1/13.1, and also catalog number 11814460001) was used to carry out immunoprecipitation from HEK293T for mass spectrometry analysis to identify nucleolus as a phase-separated protein quality control entity . Rat anti-phospho-RNA polymerase II (no. Mutations in Leucine-rich repeat kinase 2 (LRRK2) cause Parkinson's disease (PD). Histone acetylation ChIP was carried out and data were analysed as described previously (Qesta et al., 2016). Anti-GFP antibody . Total proteins were extracted from the 6-day-old . Neutralization of ACBP by suitable antibodies reduced multiple obesity-related aberrations including increased nutrient uptake, stimulated lipo-catabolism (lipolysis, triglyceride breakdown, fatty. Immunodetection using a mouse monoclonal anti-green fluorescent protein (GFP) antibody (Cat. We therefore checked each localization and activation of Aurora B. The bands are mostly present under steady-state conditions (time point 0 in the PROPEP1-YFP immunoblots) and become more abundant after damage (fig. 11814460001RocheAnti-GFP,,,! Mouse anti-tubulin (no. Documents. Blue arrowhead shows Zip1-GFP. You can also re-use the antibody . 6 answers. and 41.5 l of 20% SDS and 5 l of Proteinase K (Roche Life Sciences) were added, and the cells were . 11814460001) for 2 h at 4C before adding Protein G magnetic beads (Invitrogen, 88847). After several rinses in PBS, coverslips were incubated with a mixture of rabbit polyclonal anti-GFP (1:500, Thermo Fisher Scientific, A6455) and monoclonal anti-Crmp2 (1:100, Novus Biologicals, NBP1-50580) antibodies, rabbit polyclonal anti-GFP and mouse anti-GFP monoclonal (1:50, MilliporeSigma, 11814460001 ROCHE) antibodies (positive control . C) Representative time-lapse images of an atg1-as cell undergoing synchronized meiosis in the presence of 1-NM-PP1. We will do our best to provide everyone who is in need with the appropriate information and support. In Drosophila, this junction comprises hemi-adherens junctions that are formed upon arrival of muscles at their corresponding tendon cells. S2G). Scale Bar: 5m. 11814460001; Roche) was performed with the SuperSignal chemiluminescent detection system (Pierce, Dreieich, Germany) and a FluorChem2 MultiImager (Biozyme, Hessisch Oldendorf, Germany). e Interaction of Myc-RAD52 and GFP-CSB-AD by anti-GFP Co-IP in Flp-in 293 cells. ab72001; Abcam, Cambridge, UK), a mouse monoclonal anti-histone H3S10ph (cat. Anti-GFP antibody raised in mouse (Roche #11814460001). After washing with IP buffer, the IP samples were eluted in a reducing buffer, followed by SDS-PAGE and immunoblot analyses using anti-GFP (11814460001, Roche), anti-Flag (F1804, Sigma-Aldrich), anti-His (D291-7, MBL) antibodies. Qualit Anti-GFP is tested for functionality and purity relative to a reference standard to confirm the quality of each new reagent preparation. We found {{ ctrl2.mentions.total_count }} mentions in open access literature. View full usage report. For anti-Myc immunoprecipitation, 30 l anti-c-Myc agarose beads were added to each lysate. pkg of 50 columns, Roche: 11814460001: Anti-GFP from mouse IgG 1 (clones 7.1 and 13.1) 11814524001: rGFP Green Fluorescent Protein from jellyfish Aequorea victoria, recombinant expressed in E. coli: 11814770001: DNA Isolation Kit for Cells and Tissues sufficient for 10 isolation(s) 11815016001: Anti-HA Affinity Matrix from rat IgG 1: 11815024001 Selective autophagic degradation of mitochondria (mitophagy) is important in maintaining proper cellular homeostasis. anti-GFP (11814460001; Roche), anti-PGK (459250; Thermo Fisher Scientific, Waltham, MA), and anti-hemagglutinin (HA; 16B12; Covance, Princeton, NJ) antibodies were used for Western blotting. 11814460001; Roche), a rabbit anti-HTR12 (A.thaliana CENH3 antibody, cat. The SPATA33 protein localizes on mitochondria via its binding of the carboxyl terminal with the outer mitochondrial membrane protein VDAC2. As an example, labeling of SEP-tagged NMDA receptors can be performed with 2 L of mouse anti-GFP IgG1 (clones 7.1 and 13.1, Roche, #11814460001) or rabbit anti-GFP IgG2a (clone 3E6, Molecular Probes, # A-11120) monoclonal antibodies mixed with 2 L of QD655 goat F(ab)2 anti-rabbit IgG (Invitrogen, #Q-11421MP) or QD655 goat F(ab)2 . Anti-GFP immunoprecipitation combined with mass spectrometry revealed these bands (fig. (4) Interactions between the anti-GFP and the GFP of the cargo (transmembrane or secreted) allow the capture of the cargo and provide a picture of the history of the . Immunoprecipitation, Western blots, Immunostaining. In addition to conferring to the nucleus its shape and mechanical stability, NL is implicated in key nuclear functions including chromatin organization, transcription, DNA replication, and repair (Burke & Stewart, 2013; Dechat et al., 2008; Dittmer & Misteli, 2011). AB6556 affinity purified rabbit polyclonal). IP was conducted using the anti-GFP antibody (Roche, 11814460001, Lot: 19958500) and Pierce Protein G magnetic beads (ThermoFisher, 88847, Lot: SI253639) were used for IP. Stable and destabilized GFP reporters to monitor calcineurin activity in Saccharomyces cerevisiae - INTRODUCTION The Ca2+/calmodulin-dependent serine/threonine phosphatase calcineurin (CN) is a central component of Ca2+ signaling across species. . Anti-GFP (1 : 2000; 11814460001; Roche) and Anti-Flag (1:5000; M185-3L; MBL, Nagoya, Japan) and anti-mouse IgG (1 : 75 000; A9044-2ML; Sigma) antibodies were used for the detection of GFP-tagged, Myc-tagged and Flag-tagged . Usage and Citation Metrics . Incorporated Alk12 was detected by streptavidin-HRP western blotting. OsVQ25 - GFP was co-transfected with OsPUB73 - HA, OsPUB73 - C-HA, or NLuc - HA in rice protoplasts, followed by immunoblotting. Scale bar: 5 m. . Roche has established a multi-lingual global hotline (tel: +36 146 182 58; WhatsApp/SMS +36 707 177 394) and an email address (patientcare@roche.com) for patients and healthcare providers in or from Ukraine. For life science research only. Following two rounds of centrifugation at 16 000 g for 10 min at 4C to remove the insoluble material, the supernatants were divided into two fractions and incubated with or without anti-GFP antibodies (Roche, Cat. SNRPD3 (ab111094) and rabbit anti-NHP2L1 (ab95958) were obtained from Abcam. (Spoel et al., 2009) N/A (1:1000) Antibody: Rabbit polyclonal anti-GFP (ChIP grade) Abcam: Cat# ab290 (1:500 for ChIP) Antibody: Mouse monoclonal anti-Ubiquitin (FK2 . Mouse anti-GFP (Roche, 11814460001), rabbit anti-Mad1 10, rabbit anti-Mad2 36, mouse anti-HA (Roche, 12CA5) or rabbit anti-Cdc2 (Santa Cruz, SC-53) were used as primary antibodies. Secondary anti-mouse HRP-linked antibody (Sigma A3562). The nuclear lamina (NL) is an essential component of metazoan cells. Mixture of two high-affinity monoclonal antibodies selected for their performance in detection of GFP and GFP fusion proteins. 11814460001 Roche Anti-GFP GFP 10. A + B + C, A + B, and A denote unprocessed protein, the. Description. Manufacturer: Supply Solutions SIGMA 11814460001 Roche - Anti-GFP; from mouse IgG1? Purity: Both Anti-GFP mouse monoclonal antibodies (Clones 7.1 and 13.1) are >95% pure as determined by SDS-PAGE and ion-exchange HPLC analyses. The 35S: . no. 12. (Sigma-Aldrich Cat# 11814460001, RRID:AB_390913) Copy . In western blotting analysis, the total protein extract was probed with anti-GFP antibody for detection of the released target protein. The relative transcript levels of OsVQ25 and ACTIN were detected by RT-PCR. Mouse anti-tubulin (no. Autophagy flux . . ARIH1 is recruited to the cytosolic S. Typhimurium, localizes with LRSAM1, and contributes K48-linked polyUb chains to their Ub coat. Mouse monoclonal anti-GFP MAB3580 antibody is a popular choice [82, 226]. unknown Characterized targets eGFP ( Aequorea victoria) Host goat Clonality polyclonal Purification affinity Antigen description Antisera raised against a peptide from the GFP protein. Question. Mouse monoclonal IgG 1 clones 7.1 and 13.1 Roche (#11814460001) 1:5000 Back to protocol page . no. A. HeLa cells were infected with cytoGFP sifA S. Typhimurium for 2 h followed by fixation, immunolabeling with anti-Ub (FK2) and anti-ARIH1 antibodies, and confocal microscopy. The anti-GFP western blot shows relative protein levels. Roche11814460001 Anti-GFP 200 g. Has anyone used the Roche anti-GFP Antibody (Cat#11814460001) to detect sfGFP on a western blot? After a 12h incubation at 4C and washing for 15min in 19 PBS, the slides . HRP, Alexa-488 and Alexa-647 tagged secondary antibodies were purchased from Jackson Immunoresearch Laboratories. Product Specs Item Anti-GFP Company Roche Catalog Number 11814460001 My secondary antibody is antimouse IgG HRP conjugated This antibody. Blue arrowhead shows Zip1-GFP. (3) Addition of biotin allows the trafficking of the GFP-RUSH cargo. Submitted by However, the precise function of LRRK2 remains unclear. 04-1571) was from Merck Millipore. The MTJ mainly comprises muscle-specific PS2PS integrin receptors and their tendon-derived extracellular matrix ligand Thrombospondin (Tsp). 04- 1571) was from Merck Millipore. Antigen sequence #11814460001 Roche . The full-length TaLAX1 coding region was cloned into pGWB5-GFP to generate the 35S:TaLAX1-GFP construct. T-9026) was purchased from Sigma-Aldrich. Data and Source Information . sc20044) was purchased from Santa Cruz Biotechnology. 50-100-3387. Mouse anti-Cyclin D1 (no. Article Snippet: Roche anti-GFP (cat#11814460001), Sigma M2 anti-FLAG were used as primary antibodies, Pierce peroxidase-conjugated goat anti-mouse IgG antibody (cat#31430) and Pierce SuperSignal West Pico Extended Duration chemiluminescent detection kit (cat#34076) were used. You can generally start at 1:10, 000 and go up to 1:100, 000 depending on your expression. GFP (Roche Cat# 11814460001, RRID:AB_390913) monoclonal antibody. Probing with secondary antibodies and exposure were done as in the colony overlay assay. We report an interaction between LRRK2 and VPS52, a subunit of the Golgi-associated retrograde protein (GARP) . (2) The GFP-RUSH cell line is seeded on the coverslip. no. Catalog No. Mixture of two monoclonal antibodies, supplied as a white lyophilizatecontaining 200g of total Anti-GFP IgG. (11814460001) Anti-GFP Sign in or register to save this reagent to your favourites Antibody info Additional info Supplier MilliporeSigma Host Mouse Type Primary Clonality Monoclonal (13.1) Conjugate Unconjugated Target Green fluorescent protein UniProt P42212 - GFP_AEQVI Gene GFP Modification Unmodified View Product On Supplier's Website 1 INTRODUCTION. d Nicotiana benthamiana was used to transiently express Pto - YFP fusions encoding the WT protein or a G2A mutant. By developing the instruments and reagents you rely on each day, we strive to support your next breakthrough so you can focus on what you do: discovery. GFP 1-10: Anti-GFP N-terminal Rabbit polyclonal Sigma (#G1544) .
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